Who is Playing the Main Villain ?

Good question. I think there is a villain in the present, played by Jeremy Irons, and a villian in the past who can be clearly seen in the first trailer. But I hope the movie makers found some clever way to connect the characters, creating one storyline that involves two time periods.

In the present timeline it is either Jeremy Irons or Charlotte Rampling or Marion Cotillard. Irons and Cotillard plays the chief people at Abstergo, with Irons also portraying one of the high-ranking members of the Templar Order, while Rampling plays some powerful woman in Templar Order as well, it is known that her power can be equated to Irons' character.

In the past it definetely is Javier Gutiérrez, who plays the Grand Inquisitor himself.

Nolan, I love you forever!

I thought it was Jeremy Irons?

What's missing in movies is same as in society: a good sense of work ethic and living up to ideals.

Since this will be a trilogy, there should be a villain for entire franchise for present day. It was said that past stories might happen in other period, so I don't see a historical villain for the whole saga. Probably Thomas de Torquemada will be killed in the movie, since he will be Aguilar's main goal. But other historical villains should come with next movies.

Based on the trailer, the villains seem to be Torquemada and Irons but I think Cotillard is the main one. She gives "good girl who's actually evil" vibes.